Evaluation of deletion duplication mutation of p53 gene in gastric cancer patients in Kerman city by MLPA technique

Abstract Background: Gastric cancer (GC) is a heterogeneous disease driven by multiple genetic and epigenetic aberrations. Tp53 gene is commonly mutated in GC and correlation ofp53 mutation with clinicopathological features is contraversial. The aim of this study was firstly detection of deletion/duplication mutations in T P53 exones using multiplex ligation-dependent probe amplification (MLPA) method in GC patients and, Secondly, we investigated p53 mutation relationship with GC clinicopatholoic features. Methods: 60 GC patients were enrolled in this study and all 11 Tp53 gene exones were assessed by MLPA method. Results: most mutation was occurred in exon 1.p53 mutation was found most frequently in GC with lymph node metastasis (p:0.032) and differentiated GC (P:0.04) . Conclusion: Regarding most mutations were located in exon 1,this exone may play important role in GC carcinogenesis. More common p53 mutation in GC with lymph node metastasis indicates GC with p53 mutation may need intensive adjuvant chemotherapy or frequent follow up to prevent relapse.