تعیین نوع کاست کروموزومی ژن mec در ایزوله‌های استافیلوکوکوس اورئوس مقاوم به متی‌سیلین جمع‌آوری شده از نمونه‌های بالینی بیمارستان‌ها در منطقه سیستان

نوع مقاله : اصیل پژوهشی

نویسندگان

1 کارشناس ارشد، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه زابل، زابل، ایران

2 دانشیار، گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

چکیده

مقدمه: امروزه ظهور سویه‌های استافیلوکوکوس اورئوس مقاوم به متی‌سیلین (Methicillin-resistant Staphylococcus aureus یا MRSA)، به عنوان یکی از عوامل بیماری‌زای مهم دخیل در عفونت‌های بیمارستانی مورد توجه قرار گرفته است. تیپ‌بندی مولکولی باکتری‌ها، بخش مهمی از مطالعات اپیدمیولوژیک عفونت‌های بیمارستانی به شمار می‌رود. مطالعه حاضر با هدف تیپ‌بندی و افتراق MRSA با استفاده از روش تعیین کاست کروموزومی استافیلوکوکوس ژن mec (Staphylococcal Cassette Chromosome mec یا SCCmec) انجام شد.
شیوه مطالعه: این پژوهش بر روی 72 ایزوله MRSA انجام گردید. بدین ترتیب، پس از بررسی‌های فنوتیپی و با استفاده از روش Multiplex polymerase chain reaction (Multiplex PCR)، انواع کاست SCCmec مورد شناسایی قرار گرفت.
یافته‌ها: تیپ‌بندی SCCmec نشان داد که از میان 72 ایزوله استافیلوکوکوس اورئوس، 5 درصد حاوی تیپ SCCmec I، 45 درصد حاوی تیپ SCCmec II، 30 درصد حاوی تیپ SCCmec III و 20 درصد حاوی تیپ SCCmec V بودند.
نتیجه‌گیری: بر اساس نتایج به دست آمده، ایزوله‌های MRSA حامل انواع II و III SCCmec که شاخص سویه‌های اکتسابی از بیمارستان می‌باشند، غالب بودند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Methicillin-Resistant Staphylococcus Aureus Isolates Collected from Clinical Samples in the Sistan Region, Irann the Sistan region

نویسندگان [English]

  • Akram Vafadar-Nejad 1
  • Ahmad Rashki 2
  • Mohsen Najimi 2
1 Department of Biology, School of Basic Sciences, Zabol University, Zabol, Iran
2 Associate Professor, Department of Pathobiology, School of Veterinary Medicine, Zabol University, Zabol, Iran
چکیده [English]

Background: Today, the emerged methicillin-resistant Staphylococcus aureus (MRSA) is considered as one of the most important pathogens involved in hospital-acquired infection. Typing of bacteria is an important part of epidemiological studies on nosocomial infections. In this study, Staphylococcal Cassette Chromosome mec (SCCmec) analysis was used to identify types and differentiates MRSA clinical isolates collected from clinical samples in Sistan Region, Iran.
Methods: In this study, seventy-two MRSA isolates were obtained and the presence of SCCmec cassette chromosome mec (SCCmec) types was investigated using multiplex polymerase chain reaction (PCR) technique.
Results: Of 72 tested MRSA isolates, 5% had SCCmec type I, 45% SCCmec type II, 30% SCCmec type III, and 20% SCCmec type V.
Conclusion: According to the results of this study, SCCmec types II and III, which are the characteristics of hospital-acquired MRSA, were the most dominant types.

کلیدواژه‌ها [English]

  • Methicillin-resistant staphylococcus aureus
  • Hospital infections
  • mecA protein
  • Staphylococcus aureus

مراجع

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پژوهش در علوم بالینی
دوره 2، شماره (4-3)
مهر 1396
صفحه 117-123

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